CJF organized the studies, performed all microarray and immunohistochemistry assays, conducted and statistically analyzed QRT-PCR assays, as well as compiled and interpreted the data collected and generated the primary draft manuscript.
Microarrays assay conducted in the minor salivary glands (MCGs) of healthy controls indicated that miRNA expression profiles can be available to distinguish the glands of SS patients from those of controls and also to distinguish subsets of SS patients with low- or high-grade inflammation.
Cross-species microRNA microarray assays were conducted as described previously (Hernandez Bort et al. 2012).
VMM and CPP conducted cDNA microarray assays and preliminary analysis.
NK performed all microarray, ELISA and Western assays, conducted and statistically analyzed QRT-PCR assays, as well as compiled and interpreted the data collected and generated the primary draft manuscript.
WS and LL conceived and designed the study, conducted the siRNA transfections, RNA extraction for expression profiling, performed the microarray assays, flow cytometry and immunohistochemistry assays and PCR validation, and wrote the manuscript.
Then, the Pearson correlation coefficient of the Real-time PCR assay and the microarray assay was conducted and the value in down-regulated miRNA dateset is 0.7955, in up-regulated miRNAs dateset is 0.8889 (Figure 6, B).
One microarray assay was conducted for each PHx sample and one for its control, for a total of 32 arrays.
The microarray assays were carried out by Takara Bio. Inc. (Otsu, Japan) using products for microarray analysis manufactured by Agilent Technologies, according to the manufacturer's protocols.
We first confirmed the expression pattern of DKK genes obtained with the microarray assay by conducting a Real-time RT-PCR analysis.
