In this work we report for the first time a concerted study on gene expression in early responses to chilling and salinity using a fluorescence microarray assay based on organ-specific unigenes in sunflower.
An easy-to-handle microarray assay based on the cost-effective ArrayTube™ platform has been designed for the rapid and unequivocal identification of Coxiella burnetii, the causative agent of Q fever.
Arrayed primer extension (APEX [ 8, 9]) is a minisequencing microarray assay based on a two-dimensional array of oligonucleotide probes that are immobilized, via their 5' ends, on a glass surface.
For instance, proliferation is a predominant component of both the Oncotype Dx test (five out of 16 genes are proliferation markers) [ 33] and the MammaPrint® microarray assay based on the 70-gene prognosis signature [ 7].
To verify the anti-cancer effects of oridonin via a miRNA-dependent mechanism, comprehensive miRNA expression profiling of oridonin-treated BxPC-3 human pancreatic cancer cells was performed using a miRNA microarray assay based on Sanger miR-Base Release 20, followed by a validation using real-time PCR.
In addition to microarray-based assays, high-throughput AEI assays based on PCR and second-generation DNA sequencing have also recently been described [ 40- 42].
Based on our microarray assays, we identified 63 genes that were differentially regulated between 3937 and WPP96.
The Kyoto Encyclopedia of Genes and Genomes analysis based on mRNA microarray assays showed that the MAPK signal pathway might be involved in the inhibition of miR-135a-5p-induced miR-135a-5p-induced miR-135a-5p-induced miR-135a-5p-induced
In addition to ompA sequence alignments and BLAST search, a DNA microarray genotyping assay based on the ArrayTube™ technology was used to determine the genotype of the C. psittaci strains according to the procedures described by [ 18].
Because of its relatively lower price, higher base coverage, and greater precision with measuring transcripts compared with hybridization-based microarray assays, RNA-seq is particularly suited for the detection of transcripts, which correspond to existing genomic sequences (Wang et al. 2009).
