As compared to the original SSH and microarray combination assays, the modified SSH/microarray assays allowed for much easier inspection of the subtraction efficiency and identification of genes in the subtracted amplicons without tedious and inefficient sequencing work.
The microarray assays were carried out by Takara Bio. Inc. (Otsu, Japan) using products for microarray analysis manufactured by Agilent Technologies, according to the manufacturer's protocols.
Probing microarray assays in the presence of a hybridization mix retrieves precious information on hybridization kinetics.
Microarray assays could be informative in this regard.
VMM and CPP conducted cDNA microarray assays and preliminary analysis.
This oligonucleotide microarray-based assay allows for the detection of exonic deletions or duplications of 105 genes currently tested in our laboratory.
The gene profiling technology based on microarray assays will hopefully allow for a better selection of candidates for systemic adjuvant treatment and for individualized chemotherapy.
Importantly, this assay allowed quantification of differential mRNA expression for many candidate disease genes entirely missed in previously published microarray-based studies of mRNA expression in human brain.
All samples were used for microarray assays after adjustment to a minimum concentration (50 ng/μl).
The authors thank Tomomi Fukuda, Hiroko Tadokoro, Tatsuya Suzuki, Makiko Ichikawa, Junpei Kawauchi, Satoshi Kondou, and Kamakura Techno-Science Inc. for performing the microarray assays.
EW performed the microarray assays.
