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The oligo microarray assay yielded higher correlation coefficients with the protein data than the cDNA microarray assay, probably because the oligo probes had higher specificity, in accordance with expectations due to the lower degree of cross hybridization that generally occurs when shorter probes are used.
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The real-time Q-RT PCR assay yielded very reproducible results that supported the microarray data.
However, the TBcID assay yielded seven false negative results.
Each assay yielded a single product of the predicted size.
The two assays yielded similar results.
Approximately 25% of the conditions assayed yielded microcrystals.
Three independent DNA extractions and assays yielded the same results.
Repeated assays yielded identical results for each pair.
The microarray assay has recently emerged as a promising alternative for environmental pathogen monitoring.
More importantly, our microarray assay revealed that overexpression of G9a significantly reduced Kcna2 RNA expression in the DRG, which was further confirmed by our quantitative RT-PCR and Western blot assays.
Thus, these modifications result in a specific, sensitive, and rapid microarray assay and may be used for constructing microarrays for the detection of all influenza subtypes and strains.
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