We then compared the three multiple epitope presentations in a microarray assay using sera samples from milk allergic patients, confirming that a multiple presentation, in particular that of the tandem construct, allows for a more efficient characterization of IgE-binding fingerprints at a statistically significant level.
Similar results were obtained from the microarray assay using EFT and NB tissue samples in the Gene Expression Omnibus database GEO (Accession number: GSE1825) (data not shown).
MiRNA microarray assay using Illumina sentrix array matrix (SAM) was performed according to manufacturer's instructions (Illumina, CA) using 59 MS patients (24 RRMS, 17 SPMS and 18 PPMS) and 37 healthy controls.
Furthermore, we found no methylation changes using the "open" unbiased method of MeDIP and microarray assay using NimbleGen's Rat CpG Island Promoter microarray, which covers 15,809 islands and 14,490 promoters.
Experimental results of DNA microarray assay using genomic DNA as a reference correlated well to those obtained by a direct hybridization between two cDNA samples.
Total RNAs extracted from vaccine ILTV infected chicken embryo lung cells at 1, 2, 3 and 4 days post infection (dpi), compared to 0 dpi, were subjected to microarray assay using the two color hybridization method.
Of these 67 detected miRNAs, 27 miRNAs belonged to the 31 predicted miRNAs and 12 miRNAs were miRNA*s; another 28 miRNAs were detected only in the microarray assay using probes designed to detect known miRNA from other species.
In addition, the FI value of each protein probed with individual sera was interpolated with the calibration curve, which minimized variability in this quantitative determination so as to improve the within-slide and between-slide analytical precision.The individual rSEPs were analyzed by both immunoblot and microarray assay using the paired sera from patient 8.
To assess validity and sensitivity, we compared allele frequency estimates from microarray assays using pooled DNA to individual genotyping.
