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The obtained results confirm that the microarray assay is able to detect specifically different spoilage microorganisms present in mixture in contaminated wines.
Microarray assay is a popular and reliable method of profiling lncRNA expression.
Despite a number of publications on its application, the use of genomic DNA as a reference for microarray assay is still not wide spread.
These results could be expected since the microarray assay is performed on aliquots derived from blood culture bottles, whereas the multiplex PCR is performed on microbial DNA extracted from a different whole blood sample than the cultured portion.
Conversely, if the microarray assay is sufficiently accurate and shows individual differences between samples, then each gene will exhibit unique tendencies with respect to the stability of expression levels.
A typical NP-based genomic microarray assay is performed by directly hybridizing purified viral RNA to the capture oligos attached to the slide and then introducing intermediate oligos with a poly-A tail to form a sandwich complex (Fig. 1, Step 1).
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Microarray assay was performed on a custom array by a service provider (LC Sciences).
A microarray assay was performed using a service provider (LC Sciences, Houston, TX).
Microarray assay was performed using a service provider (LC Sciences, Houston, Texas).
The estimated reaction cost of microarray assay was eight times lower than that of available commercial methods in Brazil.
The microarray assay was able of consistently determining genotype which matters on treatment planning and was the main goal of this study.
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