Sentence examples for microarray assay including from inspiring English sources
More detailed protocols regarding the microarray assay, including microarray preparation, labeling of the cDNA probe, sample hybridization and washing can be obtained from the dbZach website [ 50].
In 25 patients, the same bacteria were identified by microbiological culture and by PCR and microarray assay (including 3 cases where PCR detected only the methicillin-resistance marker gene mecA of staphylococci).
There were 12 additional ORFs that met the 3-fold cutoff for up-regulation by σ in the microarray assay, including genes for pilin biosynthesis (hofB), histidine ammonia lyase (hutH), bEBPs (ygaA, fhlA), propanediol utilization (pduG), siderophore production (iroD), and cell invasion (invG).
Other HrpL up-regulated genes identified in the microarray assay included those with functions related to regulation and chemotaxis, as well as others with unknown function (Table 1; Supplementary Fig. S1).
Finally, each set of experiments consisted of three independent microarray assays, including one GST replicate (negative control) and two replicates of each of the on-chip enzymatic assays using the corresponding PKMT on the protein array For SETD6, the set of positive hits was considered to be the union of the sets of positive hits for each of the two PKMT protein array replicates.
Given the minimum of biological material required, also attempts have been made to exploit the benefits of microarrays for cellular assays including the detection of MHC-specific T-cell responses.
The genes that are up-regulated in macrophages by hypoxia with a probability of 100% are listed in Table 5. 14 out of 17 genes are known to be up-regulated by hypoxia or they have been validated by independent assays including microarray with other platforms.
My own 23andMe assay included the same Alzheimer's result that Kenneth Britten's did.
To confirm the differential expression of genes with low expression identified by the SSH/microarray assays (including 446 and 255 up- and down-regulated genes, respectively), we quantified the relative expression level of nine randomly selected genes, whose differential expression was detected by the SSH/microarray assays, in 18 pairs of HCC and the matched non-HCC liver tissues.
Obstacles include variable measurement in microarray assay, inconsistent assay platform, analytical requirement for comparable pair of training and test datasets, etc.
Several methods have been carried out to determine the expression of miRNAs in previous studies, including northern blotting, cloning, and microarray assay.
