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The liquid microarray assay described here had excellent clinical sensitivity (95%) for genotype determination.
Furthermore, refinement of the production of fusion molecules comprised of these SEPs and the reaction conditions of microarray assay described herein may lead to improve the sensitivity and specificity for the serodiagnosis of FESF.
However, using the microarray assay described here, the antibody response to un-glycosylated MUC1 was very weak and only seen in a very small number of patients (see Figure 3A).
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d23S ArrayTube Microarray assay as described by Borel et al. (2008).
The method used for the miRNA microarray assay was described previously (Yu et al, 2007).
In addition, ompA genotyping of samples showing monoinfection with C. trachomatis was conducted by using the ArrayStrip microarray assay as described (21 ).
Global transcriptional changes due to deficiency of RecQ helicases in primary fibroblast cells were investigated by microarray assay as described previously.
As a result, most research institutions are equipped with the basic microarray functionality required for the assay described here.
The assay described in [39] was followed.
The total RNA samples from whole splenocytes were sent to LC Sciences (http://www.lcsciences.com/) for the microarray assay as we described recently [47].
The cutoff value for individual rSEPs in the microarray assay was generated as described previously using Youden's index [ 13].
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