Second, we quantified IRG expression with a customized cDNA macroarray assay containing 166 IRGs, selected on the basis of previous microarray experiments.
The prognostic value of ICS was reaffirmed in two previously studied microarray assayed cohorts containing 274 node-negative and chemotherapy naive HRneg breast cancers, including 95 Tneg cases where it proved prognostically independent of Tneg molecular subtyping.
The microarray assay was performed using samples containing 5 µg total RNA, which were fractionated using a YM-100 Microcon centrifugal filter (Millipore, Billerica, MA) to isolate small RNAs (<300 nt).
At this symposium he described a high-throughput peptide microarray assay designed to discover new "readers" containing chromatin-associated domains that could specifically recognize H4K20me2.
Among the HrpL up-regulated genes identified in the microarray assay, only hrpA, hrpF, hrpJ, hrpK, hrpN, and hrpW were found to contain a hrp box sequence in their promoter regions (bit score >8.5).
More importantly, our microarray assay revealed that overexpression of G9a significantly reduced Kcna2 RNA expression in the DRG, which was further confirmed by our quantitative RT-PCR and Western blot assays.
The microarray assay has recently emerged as a promising alternative for environmental pathogen monitoring.
(A) Initial screening of miR-139-5p miR-139-5p miR-139-5pmicroarray assay, bioinformatargetredictions and the luciferase reporter assay.
Genetic diversity among rice cultivars from Bangladesh and North East India was assessed using a custom 384-SNP microarray assay.
The data provide valuable information for DNA microarray assay developers.
