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Microarray assay confirmed 15 of the miRNA families that were identified by bioinformatics techniques.
The microarray assay confirmed 15 conserved families identified with the bioinformatics techniques.
The DNA microarray assay confirmed the presence of the non-O157 STEC in the hemorrhaged tissues and these isolates have the locus of enterocyte effacement (LEE) pathogenicity island, stx1A, stx1B and hlyA.
A gene was considered as DE (or NDE) if at least one method, either PCR or microarray assay, confirmed it as DE (or NDE), and finally there are 1966 DE and 3388 NDE genes in this training set.
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Several genes previously demonstrated to be associated with malignant phenomena were identified by microarray assay and confirmed by RT-PCR.
Generally, this quantitative RT-PCR assay confirmed the microarray results.
Of all genes assayed, 16 exhibited differential expression in the Q-RT-PCR assay, confirming the microarray results in independent embryo samples.
Illumina microarray and Taqman genotyping assays confirmed the location of tag SNP rs725605 in the CA1 locus [ 11].
The 21 plausible B. mori miRNAs are listed in Table 1 of Additional file 1. Northern blotting was done based on microarray assay results to confirm the expression of B. mori miRNAs.
These assays confirmed the microarray data for eight of the ten mammary developmental genes: Areg, Caveolin 1 (Cav1), Esr1, Fgfr2, Mucin 1 (Muc1), Prlr, Roundabout homolog 1 (Robo1) and Wingless-type MMTV integration site family, member 5A (Wnt5A).
As shown in Figure 4, qPCR assays confirmed the results from the microarray platforms.
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