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We first confirmed the expression pattern of DKK genes obtained with the microarray assay by conducting a Real-time RT-PCR analysis.
However, the relatively high proportion of globin messenger RNA present in total RNA extracted from whole blood can reduce the efficacy of the microarray assay by interfering with the detection of less abundant gene transcripts [ 1- 3].
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The genotyping was accomplished by the DMET Drug Metabolizing Enzymess and Transporters) Plus Premier Pack, a microarray assay developed by Affymetrix (Affymetrix, Santa Clara, CA, USA) designed specifically to test drug metabolism associations.
Several genes previously demonstrated to be associated with malignant phenomena were identified by microarray assay and confirmed by RT-PCR.
The expression profile of miRNAs in CD4+ T cells from synovial fluid (SF) and peripheral blood of 33 RA patients was determined by microarray assay and validated by qRT-PCR analysis.
RNA samples used for microarray assay were reevaluated by RTPCR and confirmed the expression of cartilage matrix ECM genes (Figure 3B); however, histological cartilage tissue was not formed in the inner chamber of T-shaped implant (Figure 1C).
d23S ArrayTube Microarray assay as described by Borel et al. (2008).
The effectiveness of using genomic DNA as a reference in the microarray assay was predicted by simulations using the model and parameters described above.
Finally, the PI cannot be standardized across different microarray platforms, because the gene expression levels in microarray assay were measured by arbitrary fluorescent intensities rather than transcript copy numbers.
*H5N1 strain obtained from CDC (VA2701, Inactivated H5N1) Due to the lack of well-characterized standards for influenza A virus RNA, the detection sensitivity of the NP-based microarray assay was evaluated by using two different sources of viral RNA.
Therefore, gene expression profiles of gills of short-term (3 and 7 days) exposed crabs were investigated using a 4,462-feature 4,462-featuresay recently developed by Towle et al. [ 37].
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