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An easy-to-handle microarray assay based on the cost-effective ArrayTube™ platform has been designed for the rapid and unequivocal identification of Coxiella burnetii, the causative agent of Q fever.
In this work we report for the first time a concerted study on gene expression in early responses to chilling and salinity using a fluorescence microarray assay based on organ-specific unigenes in sunflower.
Arrayed primer extension (APEX [ 8, 9]) is a minisequencing microarray assay based on a two-dimensional array of oligonucleotide probes that are immobilized, via their 5' ends, on a glass surface.
For instance, proliferation is a predominant component of both the Oncotype Dx test (five out of 16 genes are proliferation markers) [ 33] and the MammaPrint® microarray assay based on the 70-gene prognosis signature [ 7].
To verify the anti-cancer effects of oridonin via a miRNA-dependent mechanism, comprehensive miRNA expression profiling of oridonin-treated BxPC-3 human pancreatic cancer cells was performed using a miRNA microarray assay based on Sanger miR-Base Release 20, followed by a validation using real-time PCR.
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We also estimated the false positive rate of the protein microarray assays based on the EMSA results.
In addition to ompA sequence alignments and BLAST search, a DNA microarray genotyping assay based on the ArrayTube™ technology was used to determine the genotype of the C. psittaci strains according to the procedures described by [ 18].
A previous 4C assay, based on microarray measurement of amplified 3C products, suggested that interchromosomal interactions did not contribute significantly to the nuclear microenvironment of the β-globin locus [23].
We describe here an HCV genotyping assay based on liquid microarray technology and including 16 hybridization probes specific for different HCV genotypes and subtypes and one set of primers for each of the two regions most frequently used for HCV detection and genotyping (5'UTR and NS5B).
To validate the accuracy of the microarray, a stem-loop qRT-PCR assay based on SYBR Green I was performed on five miRNAs selected at random.
CLART HPV2 (CLART; Genomica, Madrid, Spain) is a commercially available PCR-based genotyping HPV DNA assay, based on genotype amplicon-specific hybridization on a microarray.
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