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While these ultraspecific probe sequences, when incorporated in a microarray assay, are expected to be able to detect all of the 889 alleles considered, it is not guaranteed that a single assay could distinguish between different alleles.
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The obtained results confirm that the microarray assay is able to detect specifically different spoilage microorganisms present in mixture in contaminated wines.
Microarray assay was performed on a custom array by a service provider (LC Sciences).
A microarray assay was performed using a service provider (LC Sciences, Houston, TX).
Microarray assay was performed using a service provider (LC Sciences, Houston, Texas).
The estimated reaction cost of microarray assay was eight times lower than that of available commercial methods in Brazil.
The microarray assay was able of consistently determining genotype which matters on treatment planning and was the main goal of this study.
Then, the Pearson correlation coefficient of the Real-time PCR assay and the microarray assay was conducted and the value in down-regulated miRNA dateset is 0.7955, in up-regulated miRNAs dateset is 0.8889 (Figure 6, B).
RNA samples used for microarray assay were reevaluated by RTPCR and confirmed the expression of cartilage matrix ECM genes (Figure 3B); however, histological cartilage tissue was not formed in the inner chamber of T-shaped implant (Figure 1C).
The microarray assay was performed using samples containing 5 µg total RNA, which were fractionated using a YM-100 Microcon centrifugal filter (Millipore, Billerica, MA) to isolate small RNAs (<300 nt).
Microarray assay was performed using a service provider (LC Sciences).
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