In parallel, using a microarray approach, we observed differentially expressed (DE) genes across time, which were enriched for numerous functions, including cell migration, vascular remodeling, morphogenesis, cell communication, and inflammatory signaling.
Using a microarray approach, we observed that expression of genes involved in the control of barrier permeability, including inter-brain endothelial junctions (e.g. claudin-5, MARVELD-2), integrin-focal adhesions complexes (e.g. integrin β1, ELMO-1) and transporter systems (e.g. ABCB1, SLC2A1), are altered by pro-inflammatory cytokines.
Subsequently, real-time RT-PCR was performed to validate fifteen differentially expressed genes screened out by the microarray approach and high consistency was observed between the two methods.
Array-based comparative genomic hybridization and other microarray approaches have the potential to reveal other genes that possess tumor-suppressor properties.
We describe here, both qPCR and microarray approaches.
However, as observed in other studies (Sgroi et al, 1999; Menssen and Hermeking, 2002; Reinhold et al, 2003), the magnitude of the differences in gene expression assessed by quantitative real-time RT-PCR was greater than those observed using a cDNA microarray approach (Table 1).
In a clinical study, low levels of HIF-1α mRNA were observed in thyroid carcinomas using a microarray approach (Jubb et al. 2004).
Finally, using a microarray approach rather than a more targeted approach allowed us to observe the expression profiles of genes that, a priori, we would not have assayed, and have been able to identify potentially important new players in phase transition.
Using a microarray approach to analyse gene expression by lining cells of the air pouch, it was interesting to observe that the gene encoding histidine decarboxylase, the enzyme that synthesizes histamine, is rapidly upregulated after injection of MSU crystals [ 17].
Application of a high-density oligonucleotide microarray approach to study bacterial population dynamics during uranium reduction and reoxidation.
