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The disadvantage of DDRT-PCR vs. microarray approaches is clearly that it is a much more time consuming technique to perform.
The most widely accepted and used protocol for microarray approaches is the T7 RNA-polymerase based linear antisense RNA amplification protocol [ 3].
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While both metabonomics and microarray approaches are very different, both allowed identification of groups of animals according to their genotype.
To identify cancer related hypermethylated genes, also pharmacological unmasking expression microarray approaches were suited [ 21- 23].
Northern blotting, quantitative RT-PCR (QRT-PCR) and microarray approaches are commonly used to assess transcript abundance.
The change in expression of candidate genes that were differentially regulated by HMGA1 by microarray approaches was confirmed by qRT-PCR.
Expression profiling analyses using DNA microarray approaches are extensively used to study global changes in gene expression patterns of multiple cell types and tissues under different conditions.
We showed that in silico and Affymetrix microarray approaches were relevant and complementary in order to find new avian genes potentially involved in oocyte maturation and/or early embryo development, and allowed the discovery of new potential chicken mature oocyte and chicken granulosa cell markers for future studies.
The custom microarray approach is presented with procedural details for design and production in the context of neurobiological investigations.
For many, the DNA microarray approach is still the method of choice due to lower costs and availability.
So, indeed, our microarray approach is, in some cases, is also a measure of allelic diversity that exist among housekeeping genes.
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