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Moreover, the microarray approach provided allowed the discovery of a set of new potential chicken mature oocyte and chicken granulosa cell markers for future studies.
The successful identification of the metal binding protein, MT, is highlighted, not only due to its critical biological functions, but also because the blind microarray approach provided advantages over cloning methods that require significant sequence similarity to isolate genes.
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For example, the microarray approach provides a rapid overview of gene expression in a particular sample at a given time.
The use of both bioinformatics and microarray approaches provided information on the molecular mechanisms through which OET is driven in the hen.
Microarray approaches provide a wide overwiev of gene expression in experimental settings, which may yield biologically valid biomarkers for disease diagnostics, prognosis, and follow-up.
Compared to SSH or previous SSH in conjunction with microarray approaches, this modified approach provided us with three additional advantages: 1) easy inspection of the subtraction efficiency, 2) avoidance of tremendous sequencing work for gene identification, 3) high sensitivity for identifying the low-expression, differentially expressed genes.
A microarray approach cannot provide exact information on 5'- and 3'-ends of sRNAs and is limited by the genome coverage with oligonucleotide probes.
While genome sequencing and microarray approaches have provided important insight into the biology of prokaryotic organisms, including a number of human bacterial pathogens, identification of all genes and their transcriptional patterns remains a major challenge in all areas of biology.
The enterprise approach provided a base.
For that reason, traditional tools available for the analysis of expression microarrays are focused on a general approach, providing summarized values of all the probes in a probe set [e.g. RMA (Bolstad et al., 2003), MAS5 and PLIER].
We applied Literature Lab to lists of genes generated through microarray experiments to determine if such an approach provides biological insight.
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