Microarray approaches also revealed marked differences in the expression of specific cathepsin isoforms between day 2 and day 7 of involution [ 4].
In our microarray approach we also included known human miRNAs obtained from miRBase 13.0 allowing also a heterologous approach between human probes and porcine miRNAs.
Microarray approaches have also been done in this species to assess transcriptome differences between two early larval stages [ 4], to underline the liver transcriptomic response after cortisol administration [ 5, 6] or to identify the key genes involved in the re-epithelialization process after scale removal [ 7].
By using a genomic microarray approach, we were also able to profile expression and uncover which of the many paralogous gene systems in B. cenocepacia are active in an environment mimicking infection.
Microarray-based approaches also have attempted to identify novel genes associated with invasive disease but with limited sample sizes due to the array strategy employed [15].
The methods were the padlock probe ligation in combination with microarray detection (PPLMD I) [ 8], another padlock probe approach also combined with microarray detection (PPLMD II) [ 10], the protocol used in the dual tag microarray method (DTM) [ 17], a ligation-dependent probe amplification (LPA) protocol [ 12] and the SNPlex approach as described by Chaouachi et al. [ 15].
So, indeed, our microarray approach is, in some cases, is also a measure of allelic diversity that exist among housekeeping genes.
The approach also allows the analysis of different microarray experiments, microarray platforms, and cancer types simultaneously.
The accumulation pattern for the pyruvate decarboxylase (PDC) proteins (N255 and N305) also was detected by a microarray approach and activity assays in peach fruits [ 27, 77].
Here we describe a PCR-product baseDNANA microarray approach for examination of genome-wide transcriptional changes that also reliably detects faint changes in gene transcription.
