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However, there are many microarray applications where it is unavoidable for probes to share some sequence similarity to off-target transcripts.
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In addition, the development of features for normalization within, and between arrays is critical for all microarray applications, and is particularly relevant to the application of small focused arrays where a relatively large portion of the pathways may show changes in gene expression.
Because the sensor structure can be embedded in the plastic surfaces of microtiter plates or the glass surfaces of microarray slides, it is expected that this technology will be most useful in applications where large numbers of biomolecular interactions are measured in parallel, particularly when molecular labels will alter or inhibit the functionality of the molecules under study.
An arsenal of microfabrication capabilities developed in the microelectronic industry are leveraged towards on-chip assay applications, where integration of conductive, heating, and magnetic elements has resulted in the development of micro-PCR devices, microarray DNA chips containing thermal gradients, and dielectrophoretic and magnetic devices for cell and molecule preconcentration.
The variety of microarray applications allows for multiple, creative microarray designs and detection strategies.
Gives applications where possible.
Oligonucleotide probes are increasingly the method of choice for many modern DNA microarray applications.
Target concentration is the principal unknown quantity in microarray applications.
The T7 primer used in amplification of mRNA for microarray applications is a potential source for bias in microarray data.
This may also be true for microarray applications.
Although most microarray applications are research-use-only, this technology is increasingly being used in clinical based genomic applications [ 28].
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