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The T7 primer used in amplification of mRNA for microarray applications is a potential source for bias in microarray data.
One advantage of Fc-fusions for protein microarray applications is the identical means by which the many different linked proteins can be simultaneously attached to a solid surface, typically via interactions between the Fc-domain and surface-bound protein G/A.
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To this end, the NuGEN WT-Ovation™ Pico RNA Amplification System, which is generally intended for microarray applications, was initially used to generate large amounts of single stranded DNA from a small initial quantity of RNA using a three step process following the manufacturer's instructions.
Although most microarray applications are research-use-only, this technology is increasingly being used in clinical based genomic applications [ 28].
To date, however, microarray applications are still limited to preliminary screening of genome-scale transcription profiling or gene ontology analysis.
For this reason most microarray applications are now based on commercial platforms, which offer whole genome or specific subset (customized) arrays together with reagents and protocols for standardized use.
Since microarray applications comprise highly multiplexed assays that are run under only one condition (similar to multiplex PCR), it is desirable to equalize probe characteristics such as Tm and ΔG.
In addition, the development of features for normalization within, and between arrays is critical for all microarray applications, and is particularly relevant to the application of small focused arrays where a relatively large portion of the pathways may show changes in gene expression.
With antigen uptake occurring on the microarray, further applications are foreseen in the testing of antigen precursors that require uptake and processing prior to presentation.
The advent of microarray technology and its potential clinical applications is an important step toward tailored treatment.
However, there are many microarray applications where it is unavoidable for probes to share some sequence similarity to off-target transcripts.
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