In this report, we describe a spotted oligonucleotide microarray application for the investigation of cytotoxic memory T-cell repertoires against influenza matrix protein, M158 66 in HLA-A2 individuals.
DNA microarrays have been successfully used to study relative abundances of RNA and DNA, and in this paper we presented a microarray application for mapping insertion elements in microbial genomes.
The variety of microarray applications allows for multiple, creative microarray designs and detection strategies.
The T7 primer used in amplification of mRNA for microarray applications is a potential source for bias in microarray data.
From each experimental group, 2.5 μg of combined total RNA was used for microarray applications and 2 μg was used for real-time RT-PCR.
The substantial savings in initial array construction are likely to be especially advantageous for microarray applications in which oligonucleotide quality control is the primary consideration.
This offers further evidence for the lack of significant differences among a large number of classification methods reported for microarray applications in terms of the predictive performance[35], a conclusion also proposed by the newly-finished community-wide study, MAQC-II [24].
To this end, the NuGEN WT-Ovation™ Pico RNA Amplification System, which is generally intended for microarray applications, was initially used to generate large amounts of single stranded DNA from a small initial quantity of RNA using a three step process following the manufacturer's instructions.
This may also be true for microarray applications.
The new HyPer5 dye is a more ozone- and photo-stable version of Cy5 for microarray applications.
