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We observed no change in the levels of TGFB1 due to FGF2 treatment by microarray analysis whereas qRT-PCR showed downregulation of TGFB1 levels.
In one case (glycosyl-phosphatidylinositol-linked carbonic anhydrase VII [GenBank: DN739347.1]), the transcript was slightly, but significantly down-regulated in the microarray analysis, whereas it was up-regulated in the qRT-PCR analysis.
For example, hierarchical and k-means clustering are widely used techniques in microarray analysis whereas UPGMA [ 5, 6] has been mainly developed for processing proteomic data (e.g. mass-spectrometry data).
First evidence for a potential role of RAIDD in human cancer was discovered in a biochemical screen using mantle cell lymphomas, which detected a downregulation of RAIDD by microarray analysis, whereas others reported on RAIDD-linked multidrug resistance in osteosarcoma cells.
Cartilage-specific and bone-specific genes were found in the highest percentiles of expressed genes in the microarray analysis, whereas genes specifically related to T cells, B cells and platelets were found in lower percentiles; possibly from RNA originating from the subchondral bone marrow.
Similarly, thymidylate synthase (TYMS) was down-regulated by HDACi in both cell lines 2.7 - 3.8-fold in the microarray analysis, whereas qPCR determined that HDACi treatment induced a >30-fold downregulation of TYMS 24 h post-treatment in both cell lines.
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Thus, the induction of the AAD 4, AAD6, and AAD16 genes, as observed by both microarray analysis, were correct whereas the induction of the AAD3, AAD10, AAD14, and AAD15 genes in ORF DNA microarray and the induction of the AAD14 in Oligo DNA microarray were due to cross hybridization.
One samples was identified as 2a or 2c (2a by sequencing) and two samples were identified as 2c by liquid microarray analysis, confirmed by sequencing, whereas they were identified only to genotype level by Versant™.
For example, miR530a, miR1445, miR1446a-e and miR1447 were down-regulated during salt stress in P. trichocarpa, as measured by microarray analysis of known miRNAs, whereas miR482.2 and miR1450 were up-regulated [ 40].
For the LTK gene, RQ-PCR did not detect a difference in gene expression between probands and unrelated, matched controls whereas microarray analysis estimated a 2.3-fold increase in gene expression.
Studies in Xenopus laevis identified distinct phases of maternal, late embryonic and larval gene expression during the course of embryogenesis, whereas microarray analysis in Xenopus tropicalis identified several developmentally important maternal mRNAs that are regulated by changes in their adenylation during oogenesis and early development [ 15, 16].
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