Fifty of 53 (94%) gender-regulated candidate genes identified by microarray analysis were confirmed by real-time RT-PCR.
Importantly, the altered gene patterns identified by microarray analysis were confirmed by RT-PCR and real-time RT-PCR.
The experiments for microarray analysis were conducted in biological duplicate, and samples were collected at the time points indicated in the results section.
Some of the DEGs detected by the microarray analysis were also analyzed by RT-PCR, and the gene responses detected by the microarray analysis were confirmed by the RT-PCR results (Additional file 2: Figure S1). Figure 1 Similarity of gene responses across different fog treatments.
Single-stranded DNA (ssDNA) samples for microarray analysis were prepared by following a primer extension of amplicons in the presence of one primer.
RNA samples for microarray analysis were prepared according to the manufacturer's recommendations.
These ten genes retrieved from microarray analysis were fully confirmed in quantitative real time PCR.
Selected genes from the microarray analysis were validated by qRT-PCR.
CNVs identified by microarray analysis were validated by Multiplex Ligation-dependent Probe Amplification (MLPA).
The details of miRNA extraction and microarray analysis were published previously [31].
To examine if decreases detected by microarray analysis were demonstrable by QPCR, one Group III case, DA38, was employed.
