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Functional studies using microarray analysis verified by qRT-PCR must confirm in silico predicted annotations and provide biological information about gene products.
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The results of microarray analysis were verified by RQ-PCR.
The differential expression of genes obtained by microarray analysis was verified by SYBR Green real-time PCR.
The results from the ChIP-on-chip microarray analysis were verified by qPCR.
The expression profiles of selected genes obtained by microarray analysis were verified by gene expression analysis with real-time RT-PCR.
For nine selected transcripts, DE observed in the microarray analysis was verified by qPCR-analysis, indicating true differential expression for most probes having been identified as DE.
Gene expression patterns of the radioresistant sub-lines were studied through microarray analysis and verified by Western blotting and real-time PCR.
In conclusion, our studies with quantitative PCR analysis of selected genes identified with microarray analysis further verified differentially expressed genes in rats with UC as compared with that found in normal rats.
Altered mRNA expression levels of several genes, which were identified by microarray analysis, were verified by quantitative reverse transcription (RT -PCR using TaqMan® Gene ExpRT -PCR assays (Applied Biosystems), according to the manusingurer's insTaqMan®ns.
Genes found to be increased in uninfected males versus uninfected females are listed in Table 2. Using mRNA obtained in a separate experiment from the one used for the microarray analysis, we verified by qRT-PCR that the top five genes listed in Table 2 were more highly expressed in uninfected males compared to uninfected females (Table 2, Figure 2).
Using mRNA obtained in a separate experiment from the one used for the microarray analysis, we verified by qRT-PCR several of the genes shown in Figure 3. Cyp2e1 was the most highly expressed gene in this network with a fold increase of 1.8 (P = 9.4 × 10-4; Figure 3).
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