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Microarray analysis validated by quantitative PCR demonstrated an extensive range of pattern recognition receptor gene expression in resting N9 microglia, including Toll-like receptors, scavenger receptors and lectins.
Each of the 3 tested methods performed surprisingly accurately when amplifying from low inputs of total RNA based on microarray analysis validated with QPCR of 37 genes.
Based on the protein microarray analysis, validated in N. benthamiana, AtWRKY53 was phosphorylated when co-expressed with the AtMKK7/AtMPK7 module [ 57].
Analysis of gene expression using q-RT-PCR of the same RNA samples as used for microarray analysis validated the microarray results (Additional file 7: Table S1).
The authors used microarray analysis validated by reverse transcription quantitative real-time PCR to provide strong evidence that HOXA4 mRNA levels are reduced in human abdominal aortic aneurysms relative to control human abdominal aorta.
Taken together, microarray analysis validated the function of endothelial S1004A in angiogenesis.> Using B16BL6 tumor tissues little expressing S100A4, we stained tumor microvessels for CD31 and S100A4 and found that there are subpopulations of endothelial cells in tumors, S100A4-positive anegativetive ones.
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In patients undergoing living donor liver transplantation (LDLT), expression of A20 also significantly increased 1 hour after reperfusion, as determined by gene microarray analysis, validating the fact that A20 is part of the regenerative response in humans [20].
We will reveal differential gene expression levels between control and 3h ACC-treated Arabidopsis roots using CATMA microarray analysis, validate these by quantitative PCR analysis of some of the most altered genes and discuss the ethylene-mediated control of cell expansion.
The differential expression of nine transcripts (Table 1) identified by microarray analysis was validated by RT-qPCR and consistently confirmed for all the thirteen validations (six for M1775R vsWT, four for A1789T vsWT, three for Mut vsWT).
Microarray analysis was validated in qPCR studies using human postmortem brain tissue and CA1 sector and regional hippocampal dissections obtained from a mouse model of AD/Down syndrome (Ts65Dn mice) and normal disomic (2N) littermates.
We sought to identify genes whose levels of expression in whole blood correlate with self-reported smoking status.Microarrays were used to identify gene expression changes in whole blood which correlated with self-reported smoking status; a set of significant genes from the microarray analysis were validated by qRT-PCR in an independent set of subjects.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com