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The SAM package offers the advantage over other microarray analysis tools in that it does not presume equal variance or independence of genes (or both), scenarios that are often violated in biological systems [47].
For example, coexpression clustering one of the earliest developed microarray analysis tools [62], [63] provides an overall view of expression patterns of different genes, and greatly helps dissect and demonstrate the effect of each condition on the overall transcriptome.
Microarray Analysis Tools (developed by James Lund, University of Kentucky, USA) were used to perform functional analyses to identify biological processes and significantly changing gene classes.
Especially, a joint analysis of different microarray platforms that have been used on the same set of biological samples is hardly supported by most microarray analysis tools.
Raw RNA expression data was analyzed using the affy and limma packages of the Bioconductor suite of microarray analysis tools available in the R statistical environment [ 27, 28].
RNA expression data were analyzed using the "affy", "lumiHumanIDMapping", and "limma" packages of the Bioconductor suite of microarray analysis tools available for the R statistical environment.
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The ONCOMINE database and gene microarray analysis tool, a repository for published complementary DNA microarray data (http://www.oncomine.org) [25], [26], was searched to retrieve information on JMJD2B mRNA expression in human breast cancers.
The analysis was performed by dChip, a microarray analysis tool developed by Li and Wong [ 34].
Statistical over-representation of GO categories was tested through a hypergeometric test followed by the Benjamini Hochberg FDR correction using the microarray analysis tool GOToolBox.
Intensities for duplicate spots were merged to generate the final normalized data set, subsequently analyzed using TIGR's TMEV microarray analysis tool.
Enrichment of Gene Ontology (GO) [ 23] categories was statistically tested through a hypergeometric test followed by the Benjamini Hochberg FDR correction using the microarray analysis tool GOToolBox [ 24].
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