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Given that the whole human transcriptome was represented by the microarray analysis, this analysis was not biased toward the coverage of the microarray.
This discrepancy highlights the difference in sensitivity of the methodologies used, e.g. microarray analysis (this study) vs. quantitative RT-PCR analysis [ 47].
Apart from the technical challenges of microarray analysis this study is limited in resolution ability by the low sampling rate of circadian time series.
Due to the expense of microarray analysis, this is performed in only a limited number of cell strains (4), and selected genes will be further analyzed with |RT-PCR in a larger number of cell strains.
In conclusion, based on results from microarray analysis, this study demonstrates that TTA increases cardiac fatty acid oxidation and glycolysis as well as contractility and cardiac hypertrophy in Atlantic salmon.
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The microarray analysis in this study was performed essentially as outlined in [18].
Poole et al. (2007) was able to confirm approximately 62% of the changes observed in a microarray analysis, and this was similar to other reports in wheat [35].
Some loci which were not supported by FL-cDNA clones (CDS-ANE) were found to be active in transcription based on the microarray analysis in this study.
Microarray analysis confirmed this dysregulation: elevated expression (1.5 fold) of the LDL receptor gene was readily detected in NPC fibroblasts homozygous for the I1061T mutation when compared with normal fibroblasts (Table 1).
Data obtained from microarray analysis supports this hypothesis because we found increased expression levels of growth-promoting genes including Fgf1r, Fgf12, Igf1, Igf1r, Cyclin D2 in Hand1-DD heart.
YMB performed the microarray analysis for this study.
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