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Data was pre-processed and summarized using Microarray Analysis Suite (MAS) 5.0.
The images were processed with the Microarray Analysis Suite 5.0 (Affymetrix, USA).
The hybridized arrays were scanned, and raw data were extracted using the Microarray Analysis Suite 5.0 (MAS5; Affymetrix).
After scanning, raw expression data were converted into CEL files using Microarray Analysis Suite version 5 (MAS 5, Affymetrix).
Data was pre-processed and summarized by Microarray Analysis Suite (MAS) 5.0 and Robust Multiarray Analysis (RMA).
The data sets gathered with the Affymetrix microarray technology were preprocessed with MAS 5.0, the GeneChip Microarray Analysis Suite 5.0 software (Affymetrix).
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Significance analysis of microarrays (SAM multi-class analysis) using the TM4 microarray data analysis suite revealed 210 genes that were differentially regulated over the course of the experiment (Table S1).
Genes showing differential expression across the time points of the experiment were identified using SAM (multi-class) analysis [75] implemented in the MEV program of the TM4 microarray data analysis suite (http://TM4.org).
Quantification and normalization of the data was performed using "The Institute for Genomic Research TIGRGR) TM4 microarray data analysis suite.
Hybridized microarrays were analyzed by the Affymetrix microarray analysis software microarray suite 5 (MAS 5).
BN: Brown-Norway; GK: Goto-Kakizaki; LIMMA: Linear Models for Microarray Analysis; MAS5.0: MicroArray Suite version 5.0; RMA: Robust Multi-array Analysis; STZ: Streptozotocin; WKY: Wistar-Kyoto; MM: mismatch; PM: perfect match; qRT-PCR: quantitative real time PCR; vsn: variance stabilisation.
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