Exact(1)
The microarray analysis subsequently applied to elucidate early responses of a heat-tolerant and a heat-sensitive tomato genotype, also shows about 1% of the genes having significant changes in expression after the 2 h of stress.
Similar(59)
In this study, expression of miRNAs in the pig developing brain was investigated by microarray analysis and subsequently validated by qPCR.
We therefore performed a systematic assessment of early-phase gene expression responses to BDNF in striatal neurons using microarray analysis and subsequently studied the signaling pathways regulating BDNF responses using pharmacologic manipulations followed by QRT-PCR.
Several expression level changes, initially detected by microarray analysis, were subsequently confirmed by qRT-PCR validation.
The significant increase in JAG1 expression observed in our microarray analysis, and subsequently confirmed by qRT-PCR (Additional file 2C), together with the well documented role of the Notch pathway in the maintenance of "stemness", lead us to study the involvement of this pathway in SCM-1%KO PS growth and maintenance [ 12].
Only three Hnf4α microarray bound regions and seven histone-marked regions identified by microarray analysis were not subsequently identified using the corresponding ChIP-seq data.
Upregulation of both molecules was initially detected in the microarray analysis, but was subsequently confirmed by FACS and real-time RT-PCR respectively.
Rather, the protein fraction obtained likely represented a cytoplasmic complex of proteins in which the mRNAs identified by microarray analysis were associated and subsequently enriched relative to the input fractions.
Subsequently, microarray analysis allowed us to identify a number of potential targets of FtrA and FtrB regulation.
A number of genes that were poor prognostic markers have been identified in CRC patients (e.g., NUCKS1, BNIP3, PDGFC, OPG) using microarray analysis; their prognostic significance was subsequently assessed in surgically resected CRC subjects by mRNA and/or immunohistochemical (IHC) analyses [ 6– 9].
All RNA samples had a RNA Integrity Number higher than 9 and were subsequently used for microarray analysis.
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