RT-qPCR analysis showed that transcripts encoding an NADPH oxidase (RbohD), which were slightly induced in the microarray analysis, were significantly repressed during both CaLam and CaLas infection.
One or two genes were selected from interesting functional categories revealed by the microarray analysis as significantly differentially regulated by RE.
On the contrary, the level of peroxiredoxin 2 expression (5 fold upregulated in the microarray analysis) was significantly different in the two groups.
Similar to the studies of individuals with ID and ASD, detection of genomic alterations in MCA through microarray analysis was significantly higher than in traditional cytogenetic analysis [ 83, 84].
Furthermore, the relative fold changes determined via quantitative real time PCR were either equal to or greater than the fold change measured by the microarray analysis, and significantly different between the low and high cotinine subjects (P < 0.05).
However, previous microarray analysis detected significantly more genes with enriched expression in the stamens than the gynoecia despite the relatively similar levels of reproductive organ transcriptome diversity noted in this study.
Of the top 15 hypermethylated genes identified by the methylation microarray analysis, two significantly hypermethylated genes (NR2F2 and TFAP2C) were selected for further validation in independent samples using direct bisulfite sequencing.
We analysed 32 individual MaSCs by qrtPCR for expression of a panel of 12 genes identified by the Affymetrix microarray analysis as significantly more highly expressed in MaSCs than in the other mammary epithelial populations.
However, none of the other target genes, identified in the microarray analysis, were significantly up-regulated in tumor fibroblasts cultured with 0.5nM 1,25(OH)2D3 ex vivo, even though there was a trend towards up-regulation of CA2, IL1RL1 and DPP4.
Our microarray analysis highlighted significantly greater evolutionary diversification of RNA localization in the dendritic transcriptomes (81% gene identity difference among the top 5% highly expressed genes) compared to the transcriptomes of 11 different central nervous system (CNS) and non-CNS tissues (average of 44% gene identity difference among the top 5% highly expressed genes).
