The analysis of expressed sequence tag (EST) assemblies from tachyzoite and bradyzoite cDNA libraries, microarray analysis, and serial analysis of gene expression (SAGE) have identified several stage-specific genes [11] [13].
Several studies have taken different approaches to understand the genomics of the BBB, including microarray analysis, subtractive hybridization and serial analysis of gene expression [9], [10], [11].
To this end, we conducted serial OMICS studies, including DNA microarray analysis, integrated antibody chip arrays, and the implementation of bioinformatics algorithms designed to integrate siRNA data upon ALDH1 silencing.
The biological functions of predicted genes have to be validated by further studies using appropriate approaches such as insertional mutagenesis, serial analysis of gene expression, microarray analysis, proteomics, and metabolomics.
Several technologies are available to obtain profiles of the differentially expressed genes: representational difference analysis, serial analysis of gene expression, oligonucleotide microarrays, or suppression subtractive hybridization (SSH) [ 4], among others.
Some commonly used functional genomics approaches are microarray analysis, aptamers, nanobiotechnology, gene transfer, PCR, or polymerase chain reaction, RNAi, or RNA interference, SAGE, or serial analysis of gene expression, bioinformatics, epigenetics, two-dimensional gel electrophoresis, protein arrays, mass spectrometry, and metabolomics.
In this study, serial liver biopsies were available in these chimpanzees and were used for cDNA microarray analysis.
The two experimental technologies available for large-scale gene expression analysis are: 1) DNA sequencing-based serial analysis of gene expression (SAGE) and expressed sequence tag (EST) approaches and 2) dot-blot based microarray analysis.
For bees, microarray analysis of differences between healthy and afflicted bees may reveal the causes and provide insights for developing a cure.
Significant microarray analysis.
