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As mentioned previously, clustering methodologies can be applied to a wide range of biological study cases, such as microarray analysis, sequence analysis and phylogenetic analysis.
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It has been reported that cross-hybridization errors may happen in microarray analysis when sequence similarity exceeds 75%.
Another U-box/ARM gene (OsPUB6), which was found to be highly, expressed under drought and salt stress conditions in the microarray analysis, share sequence similarity with Arabidopsis AtPUB18.
Bentwich et al. [ 207] developed PalGrade by integrating bioinformatics predictions with microarray analysis and sequence-directed cloning.
MAP2656 was initially identified as absent via microarray analysis but sequencing of MAP 397 identified a homologue with 100% identity.
Using microarray analysis, 680 sequences were identified that were highly expressed in one or more of 6 immune tissues.
The core laboratory served as a training ground for faculty and students to learn new techniques including phenotype microarray analysis, DNA sequencing, and RT-PCR.
A previous study identified the transcription factor NR4A1 as a gene upregulated in primary breast cancer compared with normal tissue by microarray analysis and sequencing technologies.
Ensembl has made a first attempt at annotating these sequences genome-wide by producing a 'regulatory build' based on data from ChIP-Chip[ 18] and ChIP-Seq [ 19] experiments (chromatin immunoprecipitation followed by microarray analysis or sequencing, respectively).
Gene expression data generated by high-throughput expression profiling experiments, such as microarray analysis, RNA-sequencing, are known to comprise one of the effective genomic resources for functional prediction.
These findings subsequently were corroborated by immunohistochemical, immunofluorescent, and serologic assays, by additional culture isolates, and by a variety of molecular approaches, including reverse transcription polymerase chain reaction, microarray analysis, and sequencing (5 – 7, 12, 13 ).
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