Exact(23)
Transcription activity of protein-coding mRNA (FL-AE) is presumed to be higher than that of ncRNA (FL-NAE) and the transcriptional diversity has been confirmed by microarray analysis (see below).
Because fetal and adult hearts are composed of numerous cell types, including cardiomyocytes, endothelial cells, smooth muscle, fibroblasts, and many others, we isolated only primary ventricular cardiomyocytes for microarray analysis (see Methods S1).
The remaining body was used for RNA extraction and subsequent microarray analysis (see below).
All of the other proteins had corresponding positively modulated genes in our microarray analysis (see Additional file 3, Table S3).
The results showed similar expression profiles to the corresponding ones observed by oligo microarray analysis (see Figure 7).
It was this second subset of mosquitoes which were subsequently used as a control group for the microarray analysis (see below).
Similar(37)
For microarray data analysis, see the Supplemental Experimental Procedures.
Out of this subset, a total of 1606 genes could be identified as significantly differentially expressed after induction of differentiation (see Methods section, Microarray data analysis; see Additional file 2).
Using a novel way of microarray data analysis (see Data analysis section) a gene signature containing 44 probe sets was identified (Table 2), which was predictive of cancer metastasis.
A large multitude of various methods have been developed in the last decade to assess statistical significance of differential expression in microarray data analysis (see, e.g., the overview given in [ 33] and the references cited therein).
Our microarray analysis (details see below) suggested that both Or92a and Gr21a genes were significantly up-regulated in the sphinx mutant with p-value of 0.014 and 0.017 respectively.
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