Many more differentially regulated genes from the microarray analysis remain to be confirmed and expanded upon [as described elsewhere [ 17, 18]].
It also initiated case studies that successfully integrated microarray data into environmental risk assessment [ 43, 44], which indicates that microarray analysis will remain an important tool, especially in 21st century risk assessment practice.
Selection of an appropriate statistical method for microarray processing is a significant subject of scientific discussion and although microarrays have been in use for more than fifteen years, many issues related to data analysis remain unresolved.
We selected 15 genes representing different functional categories, which according to our microarray analysis increased, decreased, or remained essentially unchanged in terms of transcript abundance following salt-shock.
A total of 30 cDNA samples remaining after microarray analysis (30 patients) were used for the validation procedure.
Although the relative fold enrichment of some of the EC-restricted genes was somewhat lower than initially identified by microarray analysis, the expression in non-ECs remained quite low or absent in comparison to ECs.
This recruitment might take place on the post-transcriptional level [ 23, 67] and would therefore remain undetected in our microarray analysis.
Although it has been observed that expression of hsa-miR-125a-5p hsa-miR-125a-5p hsa-miR-125a-5p hsa-miR-125a-5py analysis, expression levels of hsa-miR-125a-3p in NSCLC remain unknown.
Equal amounts of the remaining RNA not used for microarray analysis were reverse transcribed with the iScript cDNA synthesis kit (BioRad, München, Germany).
Although this strategy has yielded limited success with certain malignancies, the hope that microarray analysis would provide prognostic data complementary to clinical staging has largely remained unfulfilled [ 9- 16].
