Based on the microarray analysis, the relative expression profiles of all 255 ESTs were performed hierarchical clustering with cluster software (version 3.0).
The microarray analysis overestimated relative changes of two genes by more than 20%, but both were still up-regulated by the qRT-PCR measurement.
By using Bm genomic DNA as reference for the in vivo RNA microarray analysis, the relative levels of the mRNAs to each other were obtained.
Towards this end, we performed whole-genome microarray analysis, examining relative expression levels in adult males and females that: 1) were variable between genotypes, 2) were variable specifically in each sex, and 3) showed either reproductive tissue or somatic expression.
Dual-channel cDNA microarray analysis of relative expression in 39 primary non-small cell lung carcinomas (NSCLC) compared to matched normal tissue was used to construct ranked lists of the most frequently and dramatically differentially expressed genes (Heighway et al, 2002).
To specifically identify ErbB-2-dependent mRNA changErbB-2-overexpressingsing C3.6 cells and their normal parental counterparts HB4a were serum-starved and stimulated with the mitogen HRG β1 for different times prior to RNA extraction and microarray analysis of relative mRNA levels.
Six imprinted genes were among the forty most differentially expressed transcripts as determined by microarray analysis, revealing the relative efficiency of such an approach in identifying novel imprinted genes.
Affymetrix microarray analysis of the relative gene expression profiles in whole E9.5 RFC1-/ vs. RFC1+/+ embryos identified 200 known genes that were differentially expressed.
In premature CAD patients, microarray analysis of the relative expression levels of platelet miRNAs showed an upregulation of six miRNAs (miR-340*, -451, -454*, -545 : 9.1, -615-5p, and -624*) andownregulationtiof miR-1280280, compared to controls.
To validate the results obtained from the HHV-4 Viruchip®, we therefore compared the normalised log 2 ratio of the median calculated by microarray analysis and the relative expression level of EBV by a real-time RT-PCR.
