The 87 genes identified in the microarray analysis reflect this estimation.
These data indicate that the microarray analysis reflects a true transcriptional profile of chemokines in SF PMN.
A statistically significant correlation was observed between microarray and qRT-PCR data (r = 0.7, p-value ≤ 0.0001), despite quantitative differences in the level of change, suggesting that the results obtained by microarray analysis reflect the actual changes in gene expression.
In order to verify that the changes in gene expression detected in our microarray analysis reflect actual changes in transcript levels, quantitative reverse-transcriptase PCR (qRT-PCR) was carried out using total RNAs samples isolated from plant tissue samples from identical treatments harvested independently.
These qRT-PCR results demonstrated that the microarray analysis accurately reflected expression levels of the miRNAs.
We therefore conclude that the results of tiling microarray analysis indeed reflect differences in the function of genes involved in mycoparasitism.
Our qRT-RTPCR results correlated well with the expression patterns noted in microarray data (R, 0.53 0.90) [Table 4], indicating that observed expression levels detected by microarray analysis likely reflect tumor differences.
In the latter case, Lck expression was greater in Comma/PDK1 cells as determined by qrt-PCR vs. microarray analysis, which likely reflected the extremely low background and basal expression in control cells as detected by qrt-PCR.
The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism.
We constructed a Venn diagram, based on the results of the stringent microarray analysis, to roughly reflect the temporal changes in the gene expression of SARS-CoV-infected 2B4 cells.
Noteworthy, the dedifferentiation process in monolayer culture primes hepatocytes for proliferation as shown recently by microarray analysis and therefore may reflect a phenotype contributing to liver regeneration [ 23].
