Sentence examples for microarray analysis quantitative from inspiring English sources

Expression studies were performed by microarray analysis, quantitative PCR (qPCR), reporter gene assays and immunoluminometric assays.

To validate the levels of gene expression found by microarray analysis, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) experiments were performed with formalin-fixed, paraffin-embedded (FFPE) tissues from an independent EAC patient group (N = 52).

To further investigate the expression levels of androgen regulated genes identified by microarray analysis, quantitative RT-PCR analysis was performed on selected targets using RNA pools derived from human prostate tissues.

Identification of up-regulated virulence genes by IVIAT has been confirmed in complementary studies, including gene microarray analysis, quantitative real time PCR (RT-PCR), signature tagged mutagenesis, and recombinase in vivo expressed technologies (RIVET), among others [9] [11].

In order to validate the expression results from the microarray analysis, quantitative real time PCR (qPCR) of independent cDNA preparations from the LMNA E161K mutant and LMNA normal hearts was conducted for the four genes in the two genomic clusters.

To confirm the gene expression profile revealed by microarray analysis, quantitative RT-PCR was carried out.

Aside from semi-quantitative RT-PCR in this study, microarray analysis and quantitative RT-PCR carried out in our previous study also showed that the expression level of hormones, such as TSHβ, POMC and GH in fetus, were relative lower than that in adult [ 11].

For all five genes that showed a significant decrease in expression in breast tumors with LOH at 16q compared to breast tumors without LOH, transcript expression was determined by quantitative reverse transcriptase PCR (qPCR) on the same RNA used for cDNA microarray analysis and quantitative RT-PCR as previously described [ 18, 22].

TLN performed B. bronchiseptica culturing, microarray design, microarray construction, microarray analysis, cluster analysis, quantitative real-time PCR analysis, data analysis, and drafted the manuscript.

Oligonucleotide microarray analysis and quantitative PCR were used to identify changes in expression in 3,921 genes, of which 2,348 were down-regulated and 1,573 were up-regulated.

They were incubated with RAW264.7 cells, and changes in the expression of genes were evaluated by cDNA microarray analysis and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR).