For instance, microarray analysis clearly pointed out the subtle genomic differences within the two S. Newport lineages, showing 0 to 29 gene differences within the S. Newport II lineage and 0 to 16 gene differences within the S. Newport III lineage (Table 4).
Our results suggest that microarray analysis of point mutations might be very useful for automated identification and characterization of pathogens and their hosts.
For microarray analysis, data points from technical replicate spots falling outside of mean ±1.2 times standard deviation were discarded for every gene.
Actual levels of secretion of MCP-1, TNF-alpha and FGF for all the controls and CHM samples are presented in Fig. 7B, C and D. Reduced levels of secretion of multiple factors corroborate the results of the microarray analysis and point to the possible impairment of the secretion process by cells expressing mutant REP-1.
From each cultivation experiment, biomass was harvested from the mid-exponential growth phase (biomass concentration 1.5 gr/kg) and used for RNA extraction and subsequent microarray analysis (time point 1; HacAWT-1; [glucose] = 5.8 g/L).
However, the comparison performed here not only helps to validate the findings of the microarray analysis, but also points to the mode of regulation of these differentially regulated genes.
Converging evidence using in vitro cell culture models, transgenic mouse models, gene signature microarray analysis and immunohistochemistry points out that EMT, amongst other mechanisms, is implicated in early steps of carcinogenesis, cancer cell invasion and metastasis and recurrence.
We decided to choose 6, 12 and 24 hours as time points for our microarray analysis, with the aim of understanding early events involved in cellulases and xylanase induction.
To gain significant new information about the interaction of GBS with amniotic fluid over time, we conducted expression microarray analysis at three time points throughout the pathogen growth cycle.
At all the time points, microarray analysis was performed in triplicate (using independent biological samples).
