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Regardless of the microarray platform, cyanine dyes (Cy3 and Cy5) are one of the most widely used fluorescent dye pairs for microarray analysis owing to their brightness and ease of incorporation, enabling high level of assay sensitivity.
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However, owing to the high resolution of microarray analysis, we were able to trace the minor changes in genomic content within these strains associated with each cluster (Table 4).
Traditional methods of genome wide microarray analysis, profiling steady-state levels of mRNA, may miss important mRNA targets owing to significant posttranscriptional gene regulation by RNA binding proteins (RBPs).
performed microarray analysis.
M.J.A. performed the microarray analysis.
Microarray analysis was performed at the Washington University Genome Center.
Microarray analysis was performed as previously described56.
For microarray analysis, the Agilent Array platform was employed.
(a) Genome-wide expression microarray analysis of PSC27.
Validation of the sensitivity and specificity of the microarray analysis.
A microarray analysis confirmed that csg gene expression was decreased.
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