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The microarray analysis only provided an indication of relative transcript levels in the mature biofilms continuously producing 10 mA current.
Therefore, we restricted the microarray analysis only on those probes that were still considered as valid (Illumina in-house data, available online).
Because of the limiting RNA amount isolated from muscle biopsies used for microarray analysis, only a few samples (6 cases) were used for confirmation with quantitative RT-PCR experiments.
In support of a potential role for BAMBI in kidney disease with glomerular endothelial and capillary damage and fibrosis, we observed significant induction of BAMBI mRNA expression by microarray analysis only in glomeruli isolated from kidney biopsies from 14 patients with progressive glomerular nephrosclerosis as compared to normal glomerular tissue [55].
On the basis of the expression levels of the genes determined by microarray analysis, only 7 of the inappropriately reprogrammed genes common to all SECNT embryo groups (Asz1, Magea5, Magea3, Xlr3a, Xlr5c, Hemt1, and Tktl1) were markedly repressed to less than 10% of the average expression levels in the controls.
Microarray analysis only allows detection of relative changes in gene expression.
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We then attempted to confirm the array findings for the leading differentially expressed genes using real-time PCR, which is thought to be more accurate than microarray analysis but can be used only to investigate a few genes at a time (Etienne et al. 2004).
These authors found that hsa-miR-483-5p hsa-miR-483-5p hsa-miR-483-5p hsa-miR-483-5p miRNA microarray analysis but also by qPCR techniques.
We conclude that depleting vRNAs from infected cell total RNA extracts is beneficial for the microarray analysis not only of cornavirus infection but probably for many other virus infections as well.
Microarray analysis revealed only 5 regulated genes in the 24-h group and 4 in the 72-h group.
Since no complete genome sequence exists for sunflower, cDNA microarray analysis could only be performed using the genome sequence of another crop.
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