Gene expression microarray analysis showed that the corresponding TF, namely GATA3, MYB and MYBL1 increased in allergen-challenged CD4+ cells (7-, 9- and 3-fold increases, respectively).
To validate the microarray data, semiquantitative RT-PCR was performed with the same RNAs as used in the microarray analysis of four randomly selected RA patients (namely nos 1, 2, 7 and 8) and one of the healthy donors (namely no. 2).
Our microarray analysis identified two differentially expressed members of ATF family, namely ATF-4 and ATF-6.
Recently, we have determined stress-regulated genes in the hippocampus, a higher limbic centre in the brain, of two inbred mouse strains with differential psycho-phenotype, namely C57BL/6J and the DBA/2J by employing microarray analysis [ 21].
Based on the results obtained in microarray experiments, two small RNA sequences were selected for further analysis, namely miR168 and the small RNA sequence annotated as miR415 in miRBase.
Changes in gene expression assessed by microarray analysis were confirmed by semiquantitative and quantitative RT-PCR for selected genes, namely CXCL10, CCL18, CCRL2 and CXCR4.
To gain a deeper knowledge on the p38 SAPK transcriptome we have performed whole genome microarray analysis using mouse embryonic fibroblasts (MEFs) treated with different p38 SAPK activators, namely osmostress, the cytokine TNFα and the protein synthesis inhibitor anisomycin.
Prior to DNA microarray analysis, we examined the expression of two commonly used house-keeping genes, namely glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and β-actin (supplementary material Table S1), in both hemispheres at 6 and 24 hours.
We acknowledge Bob Walker for his preparation of the human osteosarcoma samples for microarray processing and the microarray core facility at the national Human Genome Research Institute, namely Abdel Elkahloun, for assistance in the processing of all samples for microarray analysis.
A microarray analysis revealed down-regulation of several members of the Kallikrein-related peptidases (KLK) gene family, namely KLK5, KLK6, KLK7, KLK8 and KLK10, in MCF-10a lines with enhanced GNA13 protein expression.
