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Phenotype microarray analysis measures the reduction of tetrazolium dye (see experimental section).
Microarray analysis measures the level of mRNA, however, protein expression may be a more accurate measure of cellular function, and does not always mirror mRNA expression.
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First, standard methods of microarray analysis measure mRNA abundance, not mRNA synthesis or mRNA decay, and, therefore, do not provide any information regarding the mechanisms regulating transcript levels.
Microarray analysis measured the change in gene expression, in several tissues, at several cooling stages from 30 to 10°C vs. a control temperature of 30°C.
As with microarray analysis, quantitative measures of gene expression upon MYC-activation were calculated by comparing vehicle- and 4OHT-treated samples directly for each condition.
To independently test the validity of the differential expression signatures determined by microarray analysis, we measured the expression patterns of some of the putative biomarkers.
Some studies of myoblasts subjected to microarray analysis or measuring RNA transcription of FRG1 have not yielded any results showing an increase of FRG1 transcript [54], [55], while similar experiments with qRT-PCR done in other groups have shown an increased trend [27].
Relative expression of the miRNAs identified from microarray analysis was measured by real-time PCR.
Variable results include those results found to have no significant change by microarray analysis as measured by t-test.
The quality of RNA used for microarray analysis was measured using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), according to the manufacturer's instructions.
We set out to analyse the progression signature in 39 metachronous bladder tumours from 17 patients by Exon ST 1.0 microarray analysis to measure the intra-patient variation of the signature.
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