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GH contributed to computer software development for microarray analysis, LS and RG prepared microarrays.
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Microarray analysis of L-form versus classical colonies revealed many up-regulated genes of unknown function as well as multiple over-expressed stress pathways shared in common with persister cells and biofilms.
We also do not observe altered hepatic mTORC1 activity following RICTOR depletion, in agreement with our recent proteomic and microarray analysis of L-RKO mice (Lamming et al., 2014).
Firstly, microarray analysis was performed using 72 hr unstable L-form colonies that had already formed.
In this study we performed DNA microarray analysis of gene expression in E. histolytica cultured in L-cysteine-deprived conditions.
Utilization of the E. coli deletion mutant library allowed us to further examine genes required for L-form formation and survival identified by microarray analysis.
By RT-PCR we detected a qualitatively similar PDE mRNA expression profile for L-cells to that found previously by microarray analysis.
For example, a cDNA microarray analysis of TW induced by bending force in young Populus tremula (L).
In agreement with the microarray analysis, we confirmed that crabp1 mRNA and protein levels were highly expressed in ASC-Ls.
Therefore, a further microarray analysis was performed to evaluate if and how the progressive heart failure in rats with L-MI affected the gene expression patterns in PBMCs.
We used mRNA expression microarray analysis to study the transcriptional response to TCDD in young adult male C57BL/6 mice and L-E rats.
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