First, we perform a large gene expression microarray-based analysis incorporating the measurement of mRNA levels of ESR1 and PGR from approximately 4 K breast cancers.
Hence, methods such as edgeR (Robinson et al., 2010) and DEseq (Anders and Huber, 2010) updated microarray analysis to RNA-seq by incorporating appropriate statistical models, whereas other methodologies were developed specifically for the new technology (Roberts and Pachter, 2013; Tarazona et al., 2011; Trapnell et al., 2012).
Abbreviations: K - keratinising squamous cell carcinoma; nK - non-keratinising squamous cell carcinoma; n.d. - not determined; *- LNs of these patients were incorporated into microarray analysis, **- patients subjected to the unilateral lymphadenectomy.
For DNA microarray analysis, total RNA is reverse-transcribed, labeled by incorporating fluorescent dye into the cDNA, and used to hybridize microarray.
The FDR method has been widely applied to microarray analysis to detect differentially expressed genes, and is incorporated into popular software packages, e.g. SAM (Significance Analysis of Microarrays) and LIMMA (Linear Models for Microarray Data) in R.
On the other hand, application of various transcriptomic approaches in rice [9], [11] [13], including genome tiling microarray analysis [14], [15], has documented many novel transcripts that have yet to be incorporated into genome annotation.
For bees, microarray analysis of differences between healthy and afflicted bees may reveal the causes and provide insights for developing a cure.
Significant microarray analysis.
2.13 cDNA Microarray analysis.
(Beijing, China) for DNA microarray analysis.
