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Our microarray analysis gives new insights in IL-17 induced gene expression in primary hepatocytes highlighting the crosstalk with the NF-kappaB signaling pathway.
Altogether, our microarray analysis gives new insights how IL-17 may contribute to early inflammatory events in hepatocytes underlining again the importance of this cytokine in inflammatory diseases, and especially in inflammatory and autoimmune diseases of the liver.
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In the original "Network 1" of the Ingenuity Pathway Analysis, 32 of the 34 genes that appear were statistically up or down-regulated on our microarray analysis, giving it a score of 55.
Oligonucleotide sequences of the primers targeting five genes are listed in Additional file 4. Correlations between differential expression values obtained by RT-qPCR analysis and microarray analysis gave an R value of 0.94 indicating expression values obtained by microarray analysis are of good quality.
Since both analyses differed in scope - the microarray analysis gave results for 18,094 loci while only 6,121 different gene loci were included in the EST library of P. fastigiatum and were hit by at least one tag – comparisons were possible for 4,969 loci.
Canonical approaches to microarray analysis have been driven by the question of differential gene expression as measured by total transcripts encoded by a given gene.
Full details of the patients involved in the microarray analysis are given elsewhere [23] (and Noble 2009, submitted to Gut).
Xi and HC performed the experimence about microarray analysis and gave valuable advice related to the compound score issues.
The list of genes significant by different methods of microarray analysis is given in the Additional File 1 (AF1).
A detailed description of the microarray analysis is given in Methods section, and in a previous publication [ 22].
Overall, our microarray analysis may give a global overview of critical events occurring in CM-S mice compared to CM-R mice.
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