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Apart from science and engineering, bioinformatics is a vital tool in microarray analysis due to the vast amounts of data that are generated from a microarray experiment.
The quality of 19 RNA samples was insufficient for microarray analysis due to degradation.
Importantly, the differentiation-induced response of RUNX3 and its five putative targets in hIPCs cannot be gleaned from microarray analysis due to the absence of signal or their very low levels.
However, this approach is less used, in particular in microarray analysis, due to its high computational costs [ 243].
In order to reduce experimental biases in microarray analysis due to poor RNA quality, only RNA samples with RIN number >8 were further processed.
Results of two probe-sets designed to detect 5' exons of Kcnj9 gene were filtered out during microarray analysis due to weak hybridization signal.
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Inconsistencies between MPSS and microarray data could have been due to cross-hybridization problems in the microarray analysis or due to some MPSS tags representing several gene family members that had high sequence similarity.
The preference of hierarchical clustering in microarray analysis is due to its good performance [ 2] and, moreover, it does not require a pre-specification of the number of clusters.
However, the fluorescence intensity was not higher than the background threshold for any of these probes, indicating that mRNA concentrations of these cytokines (including IFN-γ) in infected and uninfected mucosal scrapings were too low to detect by microarray analysis, probably due to the relatively low percentage of cytokine-producing (immune) cells present in intestinal mucosa [ 6].
Thus, the relatively stable expression of IKBKAP observed in microarray analysis may be due to a weak expression that is masked within the noise signals.
Although we identified various genes involved in the formation of the central nerve system using subtractive hybridization, the signal intensity of these genes in the microarray analysis was weak due to the low concentration of mRNA from the brain.
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