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These results demonstrate that the MC3T3 cells used for microarray analysis differentiated appropriately and that the HDIs accelerated differentiation as expected from our previous studies [ 18].
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Microarray analysis further differentiated the 29 S. Newport isolates into two lineages, S. Newport II and S. Newport III. S. Newport II included 16 isolates, and S. Newport III covered 13 isolates (Fig. 3).
RNA recovered from the surface of the skin by EGIR has been quantified using ribonuclease protection assay, quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) and DNA microarray analysis to differentiate irritant from allergic skin reactions and by qRT-PCR to assess changes in gene expression in psoriatic skin lesions in response to biotherapy.
In an effort to define target genes of HDAC activity during oligodendrocyte differentiation we performed microarray analysis on OPCs differentiated for one day in the presence or absence of the HDAC inhibitor Trichostatin A (TSA).
Rather, the many microarray studies performed using obesity models have repeatedly demonstrated that microarray analysis can successfully differentiate between preadipocytes and adipocytes, the various body fat depots in humans, and the cellular response of adipocytes to exogenous compounds [11].
One application of microarray analysis is aimed at differentiating subgroups of cancers using gene expression profiling, also referred to as class discovery [ 1- 4].
Similar to our previously reported PFGE analysis for these strains (1), microarray analysis was able to further differentiate the 29 S. Newport isolates into two lineages (Fig. 3).
Our microarray analysis confirms that repression of Oct3/4 differentiates ES cells into the trophoblast lineage by showing the global similarity of gene expression patterns to those of TS cells.
As a control, the expression pattern was also confirmed for a gene that is expressed at similar levels in both types of differentiating xylem according to microarray analysis.
One of the limitations of microarray analysis is that it is difficult to differentiate the direct target genes from the secondary ones.
Microarray analysis has led to gene signatures that differentiate rheumatic diseases, and stages of a disease, as well as response to treatments.
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