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Using microarray analysis designed to detect the majority of mammalian miRNAs identified thus far, we demonstrated that miRNAs are aberrantly expressed in hypertrophic mouse hearts.
In order to extensively explore the expression of the genes involved in apoptosis by using a technique more sensitive than IHC, a microarray analysis designed to profile the expression of 87 key genes involved in apoptosis was performed in 40 out of 110 NTs, 20 with low and 20 with high MKI (Table 4).
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These studies, which combined chromatin immunoprecipitation of polycomb-bound promoters with microarray analysis, were designed to determine polycomb-binding regions across the genome.
Each oligonucleotide probe for microarray analysis was designed to consist of sequences of two adjacent exons to specifically interrogate the cDNA from corresponding mRNA sequence, but not the corresponding gene sequences or cDNA from unprocessed RNA.
PCR primers for individual genes selected after the microarray analysis were designed with Primer3 [ 27] to generate DNA fragments between 100-200 base pairs in length and a Tm of 56°C.
The complete human genome will be available in 2003, and a search of the entire genome for novel genes by oligonucleotide-based microarray analysis is designed; i.e. an attempt to predict all candidate human genes from the human genome and experimentally confirm the transcript status of the predicted regions as well as the entire region by using a oligo-nucleotide-based microarray [ 27, 28].
The microarray analysis pipeline was designed by Philip Lijnzaad, who also helped with statistical analysis of the data.
CK was involved in the chemostat cultivation of R. baltica and statistical analysis and in the biological interpretation of the data AE wrote MADA the microarray analysis tool and designed the microarrays.
To confirm expression results obtained from microarray analysis, primers were designed for 20 transcripts that were found to be differentially expressed between high and low quality 8CS embryos (Additional file 10).
and M.R.T. generated megakaryocytes and erythroblasts; C.E.v.d.S. provided critical expertise; T.W. isolated cells and RNA; F.D. provided critical statistical expertise; C.F.L. designed research and performed microarray analysis; S.T. and B.G. designed research, analyzed data, and wrote the paper; and W.H.O.
EN performed the ovary cultures and microarray analysis and network design.
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