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Microarray analysis data demonstrated that long-term potentiation (LTP) pathway was altered with down-regulation of genes against OS.
Although the expression of TDH3 was decreased under high temperature (based on the DNA microarray analysis data), the expression of FMP21 in the FMP21-overexpressing strains was higher than that in the control strains.
Microarray analysis, data normalization, and DNA binding specificity analysis were performed as previously described [20], [31].
Microarray analysis data collected at different time points (Figure S2A) show dramatically impaired differentiation capacity of Shp2−/− mESCs.
BT/HerR0.2 clones, which did not have up-regulated PPP1R1B in the microarray analysis (data not shown), were also analyzed in this experiment.
For microarray analysis, data points from technical replicate spots falling outside of mean ±1.2 times standard deviation were discarded for every gene.
The microarray analysis data can be retrieved using access number GSE4972 in the Gene Expression Ominibus database at http://www.ncbi.nlm.nih.gov/geo.nih.gov/geo
The microarray analysis data can be retrieved using access number GSE14981 in the Gene Expression Ominibus database at http://www.ncbi.nlm.nih.gov/geo.nih.gov/geo
For the gene expression microarray analysis, data are generally normalized under an assumption that the total amount of mRNA is constant between different samples.
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TB carried out the data compilation, microarray analysis, and data analysis.
BS and MK performed microarray analysis and data analysis.
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