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This was aimed at getting a sufficient statistical power which is reached by a minimum of three biological replicates per analysed point and at reducing microarray analysis cost.
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Our analysis of next generation sequencing was motivated by the rapidly converging cost of microarray analysis with a single lane of data from an Illumina 1 G sequencer.
It may also be due to the relatively high cost of performing microarray analysis.
In light of the cost associated with microarray analysis, we went on to attempt to reduce the number of predictive genes necessary to achieve both response prediction and PFS stratification using data from the Khambata-Ford et al study.
However, this approach is less used, in particular in microarray analysis, due to its high computational costs [ 243].
Minimal costs associated with microarray analysis allow for the generation of high-throughput expression profiles or combinations of profiles of elite breeding lines for accelerated crop-breeding efforts.
Protocols that require multiple rounds of amplification can accumulate material and labor costs that quickly exceed those associated with the actual microarray analysis, and amplification can become a "rate-limiting" (or "cost-limiting") factor in study design.
Electrical readout systems have emerged as an alternative to conventional optical methods for microarray analysis thanks to its potential advantages like low-cost, low-power and easy miniaturization of the required instrumentation.
Ideally, new technologies will reduce sample volume requirements, lessen the amount of time required for microarray analysis (which now averages 24 hours), and reduce microarray costs to the extent that they can be used routinely in the clinic.
These observations indicate that focused microarray analysis of PBMCs may be a practical, useful, and low-cost bedside diagnostics tool.
Additionally, due to cost limitations we chose to use one replicate of the experiment for microarray analysis and the other replicate for in situ hybridization (ISH) analysis.
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