How do the new NGF-regulated genes identified by gene microarray analysis contribute to the control of sympathetic neuron death and survival?
SEFB participated in construction of the custom expression array, performed microarray data analysis, contributed to analysis of physiological parameters, constructed the genome-scale metabolic model and performed comparisons with other species, and drafted the manuscript.
JRP performed microarray analysis and contributed to drafting the manuscript.
AL performed the initial microarray analysis and contributed to data analysis.
AA participated in the design of the study and carried out the vector construction, plant transformation and molecular analysis, microarray analysis, and contributed to the drafting of the manuscript.
MMP carried out the sampling, RNA, microarray work and analysis, and RT-PCR and qRT-PCR experiments; MJG carried out the sequence annotation and participated in microarray analysis; AA contributed in sequence analysis and in silico metabolic studies; and VP participated in sampling, microarray analysis and wrote the paper.
PJS performed library preparation, sequencing, microarray hybridisations, qPCR, data analysis, contributed to the experimental design and drafted the manuscript.
JEB supervised and advised on microarray design and data analysis, contributed to planning and supervision of the study and assisted in writing of the manuscript.
Many variables influence the outcome of the experiments in microarray analysis, thus contributing to experimental errors and biological variations (for more details see [ 233]).
The data presented here clearly indicate that microarray analysis can contribute to identify unknown mode-of-actions, as has previously been shown for the fungicide ciclopirox olamine in Candida albicans [ 50].
